Review



phosphorylated stat5  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Cell Signaling Technology Inc phosphorylated stat5
    Phosphorylated Stat5, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 461 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphorylated+stat5/Phospho-Stat5+(Tyr694)+Rabbit+mAb/pm25419444__ml500165r_si_001-72-13-16
    Average 96 stars, based on 461 article reviews
    phosphorylated stat5 - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Immunostaining:

    Article Title: Leptin on the apical surface inhibits casein production and STAT5 phosphorylation in mammary epithelial cells.
    Article Snippet: Leptin is a peptide hormone present in both the blood and milk.. A close relationship between leptin and milk production in lactating mammary glands has been previously reported.. However, how leptin influences milk production in lactating mammary glands remains unclear.

    Article Title: Effects of hydrostatic compression on milk production-related signaling pathways in mouse mammary epithelial cells.
    Article Snippet: Mammary epithelial cells (MECs) secrete milk into the mammary alveolar lumen during lactation.. The secreted milk accumulates in the alveolar lumen until milk ejection occurs, and excess milk accumulation downregulates milk production in alveolar MECs.. Intramammary hydrostatic pressure also increases in the alveolar lumen in a manner dependent on milk accumulation.

    Western Blot:

    Article Title: Leptin on the apical surface inhibits casein production and STAT5 phosphorylation in mammary epithelial cells.
    Article Snippet: Leptin is a peptide hormone present in both the blood and milk.. A close relationship between leptin and milk production in lactating mammary glands has been previously reported.. However, how leptin influences milk production in lactating mammary glands remains unclear.

    Article Title: Effects of hydrostatic compression on milk production-related signaling pathways in mouse mammary epithelial cells.
    Article Snippet: Mammary epithelial cells (MECs) secrete milk into the mammary alveolar lumen during lactation.. The secreted milk accumulates in the alveolar lumen until milk ejection occurs, and excess milk accumulation downregulates milk production in alveolar MECs.. Intramammary hydrostatic pressure also increases in the alveolar lumen in a manner dependent on milk accumulation.

    Bioprocessing:

    Article Title: Leptin on the apical surface inhibits casein production and STAT5 phosphorylation in mammary epithelial cells.
    Article Snippet: Leptin is a peptide hormone present in both the blood and milk.. A close relationship between leptin and milk production in lactating mammary glands has been previously reported.. However, how leptin influences milk production in lactating mammary glands remains unclear.

    Article Title: Effects of hydrostatic compression on milk production-related signaling pathways in mouse mammary epithelial cells.
    Article Snippet: Mammary epithelial cells (MECs) secrete milk into the mammary alveolar lumen during lactation.. The secreted milk accumulates in the alveolar lumen until milk ejection occurs, and excess milk accumulation downregulates milk production in alveolar MECs.. Intramammary hydrostatic pressure also increases in the alveolar lumen in a manner dependent on milk accumulation.

    Incubation:

    Article Title: Novel therapeutic role of Ganoderma Polysaccharides in a septic mouse model - The key role of macrophages
    Article Snippet: .. Afterward, the membranes were incubated with GAPDH (#5174), total NF-κB p65 (#8242), phosphorylated NF-κB p65 (#3033), total STAT5 (#25656), and phosphorylated STAT5 (#4322; purchased from CST, USA) antibodies. .. ChemiDoc XRS Plus luminescent image analyzer (Bio-Rad, https://www.bio-rad.com/zh-cn ) was used for imaging.

    Immunodetection:

    Article Title:
    Article Snippet: .. Immunodetection was performed using antibodies against the total STAT5 (Cell Signaling # 9363), phosphorylated STAT5(Tyr 694) (Cell Signaling, cat.# 9359), total STAT3 (Cell Signaling #4904) and phosphorylated STAT3 (Tyr 705) (Cell Signaling #9661), Cleaved Caspase-3 (Asp175) (Cell Signaling #9661), Cleaved-Parp-1 (ab72805) Anti-Cyclin D1 (Abcam ab16663), Anti-Cyclin-D2 (Abcam ab94685), Anti-c-Myc (Abcam ab32072) and β-actin (Cell Signaling #3700) as a loading control, followed by fluorescent conjugated goat secondary antibodies Alexa Fluor 488 (Cell Signaling #4408) and Alexa Fluor 647 (Cell Signaling # 4414). .. The bands were visualized using the Chemi-doc MP System (Bio-Rad).

    Control:

    Article Title:
    Article Snippet: .. Immunodetection was performed using antibodies against the total STAT5 (Cell Signaling # 9363), phosphorylated STAT5(Tyr 694) (Cell Signaling, cat.# 9359), total STAT3 (Cell Signaling #4904) and phosphorylated STAT3 (Tyr 705) (Cell Signaling #9661), Cleaved Caspase-3 (Asp175) (Cell Signaling #9661), Cleaved-Parp-1 (ab72805) Anti-Cyclin D1 (Abcam ab16663), Anti-Cyclin-D2 (Abcam ab94685), Anti-c-Myc (Abcam ab32072) and β-actin (Cell Signaling #3700) as a loading control, followed by fluorescent conjugated goat secondary antibodies Alexa Fluor 488 (Cell Signaling #4408) and Alexa Fluor 647 (Cell Signaling # 4414). .. The bands were visualized using the Chemi-doc MP System (Bio-Rad).

    Inhibition:

    Article Title: BCR::ABL1 kinase N-lobe mutants confer moderate to high degrees of resistance to asciminib.
    Article Snippet: Division of Hematology/Oncology, Department of Medicine, University of California, San Francisco, CA; Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine, Miami, FL; Department of Pharmaceutical Chemistry, University of California, San Francisco, CA; Institute for Physiological Chemistry, Philipps University of Marburg, Marburg, Germany; Adult Hematology Department, Ho ˇ pital Saint-Louis, Paris, France; and Antidote Health Foundation for Cure of Cancer, Cambridge, MA om http://ashpublications.oticle-pdf/144/6/639/2237334/blood_b KEY PO INT S



    Similar Products

    95
    Bioss rabbit polyclonal anti human phosphorylated stat5
    Silencing of MRCKα inhibits the stimulatory effects of Met and Leu on mRNA abundance of genes related to milk protein synthesis in bovine mammary epithelial cells. Cells were transfected with scrambled siRNA (control, SC) or MRCKα siRNA (SI) and stimulated simultaneously with control (B, basal medium), additional Met (M, Met = 0.6 mM) or Leu (L, Leu = 0.6 mM). After 24 h, cells were collected and isolated for RT-qPCR analysis. Values are presented as mean ± SEM for three independent experiments. P AA , P -value of amino acid addition (AA); P SI , P -value of silencing of MRCKα (SI); P AA×SI , P -value of AA × SI interaction. CSN2 = β-casein; CSN1S1 = αS1-casein; CSN3 = κ-casein; LALBA = lactalbumin α; MRCKα = myotonic dystrophy-related CDC42-binding kinase alpha; mTOR = mechanistic target of rapamycin; JAK2 = Janus kinase 2; <t>STAT5</t> = signal transducers and activators of transcription 5.
    Rabbit Polyclonal Anti Human Phosphorylated Stat5, supplied by Bioss, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphorylated+stat5/Rabbit+Anti-Human+IgGF(ab')2+Antibody/pmc12143634-50-12-39
    Average 95 stars, based on 1 article reviews
    rabbit polyclonal anti human phosphorylated stat5 - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    86
    Galectin Therapeutics stat5 phosphorylation
    Silencing of MRCKα inhibits the stimulatory effects of Met and Leu on mRNA abundance of genes related to milk protein synthesis in bovine mammary epithelial cells. Cells were transfected with scrambled siRNA (control, SC) or MRCKα siRNA (SI) and stimulated simultaneously with control (B, basal medium), additional Met (M, Met = 0.6 mM) or Leu (L, Leu = 0.6 mM). After 24 h, cells were collected and isolated for RT-qPCR analysis. Values are presented as mean ± SEM for three independent experiments. P AA , P -value of amino acid addition (AA); P SI , P -value of silencing of MRCKα (SI); P AA×SI , P -value of AA × SI interaction. CSN2 = β-casein; CSN1S1 = αS1-casein; CSN3 = κ-casein; LALBA = lactalbumin α; MRCKα = myotonic dystrophy-related CDC42-binding kinase alpha; mTOR = mechanistic target of rapamycin; JAK2 = Janus kinase 2; <t>STAT5</t> = signal transducers and activators of transcription 5.
    Stat5 Phosphorylation, supplied by Galectin Therapeutics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphorylated+stat5/pm41503909-99-18-4
    Average 86 stars, based on 1 article reviews
    stat5 phosphorylation - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    96
    Cell Signaling Technology Inc phosphorylated stat5
    Silencing of MRCKα inhibits the stimulatory effects of Met and Leu on mRNA abundance of genes related to milk protein synthesis in bovine mammary epithelial cells. Cells were transfected with scrambled siRNA (control, SC) or MRCKα siRNA (SI) and stimulated simultaneously with control (B, basal medium), additional Met (M, Met = 0.6 mM) or Leu (L, Leu = 0.6 mM). After 24 h, cells were collected and isolated for RT-qPCR analysis. Values are presented as mean ± SEM for three independent experiments. P AA , P -value of amino acid addition (AA); P SI , P -value of silencing of MRCKα (SI); P AA×SI , P -value of AA × SI interaction. CSN2 = β-casein; CSN1S1 = αS1-casein; CSN3 = κ-casein; LALBA = lactalbumin α; MRCKα = myotonic dystrophy-related CDC42-binding kinase alpha; mTOR = mechanistic target of rapamycin; JAK2 = Janus kinase 2; <t>STAT5</t> = signal transducers and activators of transcription 5.
    Phosphorylated Stat5, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphorylated+stat5/Phospho-Stat5+(Tyr694)+Rabbit+mAb/pm25419444__ml500165r_si_001-72-13-16
    Average 96 stars, based on 1 article reviews
    phosphorylated stat5 - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    86
    Huabio Inc phosphorylated stat5
    Expression of PRL/PRLR signaling pathway related proteins in liver tissue, and expression levels of PRLR (A) , JAK2 (B) , p-JAK2 (C) , STAT5a (D) , and <t>p-STAT5</t> (E) in liver tissue of mice in each group.
    Phosphorylated Stat5, supplied by Huabio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphorylated+stat5/anti+stat3/pmc12390791-79-60-66
    Average 86 stars, based on 1 article reviews
    phosphorylated stat5 - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    86
    Danaher Inc rabbit anti phosphorylated stat5
    Expression of PRL/PRLR signaling pathway related proteins in liver tissue, and expression levels of PRLR (A) , JAK2 (B) , p-JAK2 (C) , STAT5a (D) , and <t>p-STAT5</t> (E) in liver tissue of mice in each group.
    Rabbit Anti Phosphorylated Stat5, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphorylated+stat5/pm39044768-86-24-30
    Average 86 stars, based on 1 article reviews
    rabbit anti phosphorylated stat5 - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    90
    Thermo Fisher phosphorylated stat5 y694
    Expression of PRL/PRLR signaling pathway related proteins in liver tissue, and expression levels of PRLR (A) , JAK2 (B) , p-JAK2 (C) , STAT5a (D) , and <t>p-STAT5</t> (E) in liver tissue of mice in each group.
    Phosphorylated Stat5 Y694, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphorylated+stat5/anti+pstat5/us12060332-430-23-39
    Average 90 stars, based on 1 article reviews
    phosphorylated stat5 y694 - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Cell Signaling Technology Inc phosphorylated stat5 y694
    Expression of PRL/PRLR signaling pathway related proteins in liver tissue, and expression levels of PRLR (A) , JAK2 (B) , p-JAK2 (C) , STAT5a (D) , and <t>p-STAT5</t> (E) in liver tissue of mice in each group.
    Phosphorylated Stat5 Y694, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphorylated+stat5/anti+stat3/us12060332-430-23-43
    Average 90 stars, based on 1 article reviews
    phosphorylated stat5 y694 - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    Image Search Results


    Silencing of MRCKα inhibits the stimulatory effects of Met and Leu on mRNA abundance of genes related to milk protein synthesis in bovine mammary epithelial cells. Cells were transfected with scrambled siRNA (control, SC) or MRCKα siRNA (SI) and stimulated simultaneously with control (B, basal medium), additional Met (M, Met = 0.6 mM) or Leu (L, Leu = 0.6 mM). After 24 h, cells were collected and isolated for RT-qPCR analysis. Values are presented as mean ± SEM for three independent experiments. P AA , P -value of amino acid addition (AA); P SI , P -value of silencing of MRCKα (SI); P AA×SI , P -value of AA × SI interaction. CSN2 = β-casein; CSN1S1 = αS1-casein; CSN3 = κ-casein; LALBA = lactalbumin α; MRCKα = myotonic dystrophy-related CDC42-binding kinase alpha; mTOR = mechanistic target of rapamycin; JAK2 = Janus kinase 2; STAT5 = signal transducers and activators of transcription 5.

    Journal: Animal Nutrition

    Article Title: Myotonic dystrophy-related CDC42-binding kinase alpha (MRCKα) mediates methionine- and leucine-stimulated β-casein synthesis in bovine mammary epithelial cells via targeting mTOR

    doi: 10.1016/j.aninu.2025.01.003

    Figure Lengend Snippet: Silencing of MRCKα inhibits the stimulatory effects of Met and Leu on mRNA abundance of genes related to milk protein synthesis in bovine mammary epithelial cells. Cells were transfected with scrambled siRNA (control, SC) or MRCKα siRNA (SI) and stimulated simultaneously with control (B, basal medium), additional Met (M, Met = 0.6 mM) or Leu (L, Leu = 0.6 mM). After 24 h, cells were collected and isolated for RT-qPCR analysis. Values are presented as mean ± SEM for three independent experiments. P AA , P -value of amino acid addition (AA); P SI , P -value of silencing of MRCKα (SI); P AA×SI , P -value of AA × SI interaction. CSN2 = β-casein; CSN1S1 = αS1-casein; CSN3 = κ-casein; LALBA = lactalbumin α; MRCKα = myotonic dystrophy-related CDC42-binding kinase alpha; mTOR = mechanistic target of rapamycin; JAK2 = Janus kinase 2; STAT5 = signal transducers and activators of transcription 5.

    Article Snippet: Rabbit polyclonal anti-human signal transducer and activator of transcription 5 (STAT5) antibody, rabbit polyclonal anti-human phosphorylated STAT5 (Tyr 694 ) antibody, rabbit polyclonal anti-human PI3K antibody and rabbit polyclonal anti-human phosphorylated PI3K (Tyr 317 ) antibody were purchased from Bioss antibodies (Beijing, China).

    Techniques: Transfection, Control, Isolation, Quantitative RT-PCR, Binding Assay

    Expression of PRL/PRLR signaling pathway related proteins in liver tissue, and expression levels of PRLR (A) , JAK2 (B) , p-JAK2 (C) , STAT5a (D) , and p-STAT5 (E) in liver tissue of mice in each group.

    Journal: Frontiers in Pharmacology

    Article Title: Exercise ameliorates hepatic lipid accumulation via upregulating serum PRL and activating hepatic PRLR-mediated JAK2/STAT5 signaling pathway in NAFLD mice

    doi: 10.3389/fphar.2025.1647231

    Figure Lengend Snippet: Expression of PRL/PRLR signaling pathway related proteins in liver tissue, and expression levels of PRLR (A) , JAK2 (B) , p-JAK2 (C) , STAT5a (D) , and p-STAT5 (E) in liver tissue of mice in each group.

    Article Snippet: The PVDF membrane was blocked with 5% bovine serum albumin (BSA) at room temperature for 1 h and then incubated overnight at 4°C with gentle shaking in the presence of the following primary rabbit monoclonal antibodies: total PRLR (1:500 dilution, ER 1915-43, Huabio), JAK2 (1:1000 dilution, ET1607-35, Huabio), phosphorylated JAK2 (p-JAK2; 1:1000 dilution, ET1607-34, Huabio), STAT5a (1:1000 dilution, ET1610-58, Huabio), phosphorylated STAT5 (p-STAT5; 1:1000 dilution, ET1610-48, Huabio), and β-actin (1:100,000 dilution, AC026, Abclonal).

    Techniques: Expressing